北京华创恒达科技有限公司

采购指南：  

1、MET/CEN7 双色显色原位杂交探针： 

     货号：C-3057-400
     名称：ZytoDot 2C SPEC MET/CEN 7 Probe 
     标记：Digoxigenin/DNP
     规格：400μl


2、双色显色原位杂交预处理试剂盒： 

     货号：C-3044-40
     名称：ZytoDot 2C CISH Implementation Kit
     规格：40人份
     组成：Heat Pretreatment Solution EDTA, 500 ml;
                Pepsin Solution, 4ml; 
                Wash Buffer SSC, 500 ml; 
                20x Wash Buffer TBS, 2x 50 ml; 
                Anti-Dig/DNP-Mix, 4 ml; 
                HRP/AP-Polymer-Mix, 4 ml;
                AP-Red Solution A, 0.4 ml; 
                AP-Red Solution B, 15 ml; 
                HRP-Green Solution A, 0.8 ml; 
                HRP-Green Solution B, 15 ml; 
                Nuclear Blue Solution, 20 ml; 
                Mounting Solution (alcoholic), 4 ml



文献：

1、Cooper CS, et al. (1984) Nature 311: 29-32.
2、Engelman JA, et al. (2007) Science 316: 1039-43.
3、Garcia S, et al. (2007) Int J Oncol 31: 49-58.
4、Hara T, et al. (1998) Lab Invest 78: 1143-53.
5、Isola J, Tanner M (2004) Methods Mol Med 97: 133-44.
6、Mayr D, et al. (2009) Histopathology 55: 716-23.
7、Speel EJ, et al. (1994) J Histochem Cytochem 42: 1299-307.
8、Tsukamoto T, et al. (1991) Int J Dev Biol 35: 25-32.
Wilkinson DG: In Situ Hybridization, A Practical Approach, Oxford University Press (1992)
ISBN 0 19 963327 4.