Background
The ZytoLight ® SPEC HER2/TOP2A/CEN 17 Triple Color Probe is designed for the simultaneous detection of HER2 and TOP2A gene status.
The HER2 gene (a.k.a. ERBB2 and NEU) is located in the chromosomal region 17q12 and encodes a 185 kDa transmembrane glycoprotein.
The TOP2A (topoisomerase II alpha) gene is located in the chromoso-mal region 17q21-22 and encodes a 170 kDa DNA topoisomerase. The TOP2A gene is frequently either co-amplified or deleted in HER2 positive breast cancer cases. TOP2A functions as the target for several anticancer agents, e.g. anthracyclines. Recent data suggests that amplification and deletion of the TOP2A gene locus may account for relati-ve chemosensitivity or resistance to TOP2A inhibitor therapy in HER2 positive breast cancer. Thus, determination of the HER2 and TOP2A status may help to predict benefit from adj
Probe Description
The SPEC HER2/TOP2A/CEN 17 Triple Color Probe is a mixture of a green fluoro-chrome direct labeled SPEC HER2 probe specific for the HER2 gene at 17q12, an orange fluorochrome direct labeled SPEC TOP2A probe specific for the TOP2A gene at 17q21-22, and a blue fluorochrome direct labeled CEN 17 probe specific for the alpha satellite
Results
In a normal interphase nucleus, two green, two orange, and two blue signals are ex-pected. In a cell with amplification of the HER2 gene locus, multiple copies of the green signal or large green signal clusters will be observed. Amplification of TOP2A will result in multiple copies of the orange signal or large orange signal clusters. Deletion of the TOP2A gene results in a reduced number of red signals.
采购指南：
联系电话：010-64484022、64484033；400-699-7909
Z-2093-50 ZytoLight SPEC HER2/TOP2A/CEN 17 Triple Color Probe 5 (50μl)
Z-2093-200 ZytoLight SPEC HER2/TOP2A/CEN 17 Triple Color Probe 20 (200μl)
Z-2028-5 ZytoLight FISH-Tissue Implementation Kit 5
Z-2028-20 ZytoLight FISH-Tissue Implementation Kit 20
