| Background The ZytoLight ® SPEC MET/CEN 7 Dual Color Probe is designed for the detection of MET gene amplifications found in a variety of human tumors. The MET gene (a.k.a. c-Met) is located in the chromosomal region 7q31.2 and encodes a transmembrane tyrosine kinase receptor for the hepathocyte growth factor (HGF). HGF and MET play an important role in angiogenesis and tumor growth.Activation or upregulation of MET was found in a number of carcinomas inclu-ding lung, breast, colorectal, prostate, and gastric carcinomas as well as in gliomas, melanomas and some sarcomas. MET overexpression is known as negative pro-gnostic indicator in patients with various carcinomas, multiple myeloma, or glioma. Therefore, several inhibitors of the HGF/MET signaling pathway are being studied and developed as potent therapies to inhi-bit angiogenesis and tumor growth.Recently, it was shown that MET amplifi-cation leads to resistance to gefitinib or erlotinib in lung cancer by driving HER3-dependent activation of the PI3K pathway. Probe Description The SPEC MET/CEN 7 Dual Color Probe is a mixture of an orange fluorochrome direct labeled CEN 7 probe specific for the alpha satellite centromeric region of chromosome 7 (D7Z1) and a green fluorochrome direct labeled SPEC MET probe specific for the MET gene located at 7q31. Results In a normal interphase nucleus, two oran-ge and two green signals are expected. In a cell with amplification of the MET gene locusved. 采购指南 联系电话：010-64484022、64484033；400-699-7909 Z-2087-200 ZytoLight SPEC MET/CEN 7 Dual Color Probe 20 (200μl) Z-2028-20 ZytoLight FISH-Tissue Implementation Kit 20 |
