简介
The ZytoLight ® SPEC ALK/EML4 TriCheck™ Probe is designed to detect inversions involving the chromosomal region 2p23 harboring the ALK gene and the chromosomal region 2p21 harboring the EML4 gene. Moreover, using this probe it is possible to discriminate between EML4-ALK inversions and translocations affecting ALK, but not EML4, such as ALK-TGF or ALK-KIF5B translocations.
探针描述
The SPEC ALK/EML4 TriCheck™ Probe is a mixture of three direct labeled probes hybridizing to the short arm of chromosome 2. The orange fluorochrome direct labeled probe hybridizes distal to the ALK gene breakpoint region at 2p23, the green fluorochrome direct labeled probe hybridizes proximal to the ALK gene breakpoint region at 2p23, and the blue fluorochrome direct labeled probe hybridizes to the EML4 gene region at 2p21.
结果
In an interphase nucleus of a normal cell lacking an inversion involving the 2p21-
23 band, two orange/green fusion signals and two blue signals are expected
representing two normal (non-rearranged) 2p21-23 loci.
One 2p21-23 locus affected by an EML4- ALK inversion is indicated by one separate green signal, one separate orange signal, and an additional blue signal. The separate green and orange signals each co-localize with a blue signal.
采购指南：
联系电话：010-64484022、64484033；400-699-7909
Z-2117-50 ZytoLight SPEC ALK/EML4 TriCheck Probe 5 (50μl)
Z-2117-200 ZytoLight SPEC ALK/EML4 TriCheck Probe 20 (200μl)
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Z-2028-20 ZytoLight FISH-Tissue Implementation Kit (20)
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