简介
The ZytoLight ® SPEC BCR/ABL Dual Color Dual Fusion Probe is designed for the detection of the specific translocations involving the chromosomal region 9q34 harboring the ABL (a.k.a ABL1) gene,and the chromosomal region 22q11, harboring the BCR (a.k.a. BCR1) gene. Rearrangements involving t(9;22)(q34;q11)are observed in approx. 90% of patients with chronic myeloid leukemia (CML) and in approx. 25% of adults with acute lymphoblastic leukemia (ALL). The rearrangements are cytogenetically characterized by the presence of the Philadelphia (Ph)chromosome.
The translocation frequently results in the formation of a chimeric BCR/ABL fusion gene on the derivative chromosome 22.The gene product is a BCR/ABL protein with abnormal tyrosine kinase activity. In normal cells, ABL kinase activity is finely regulated in response to growth factors and other stimuli. The BCR/ABL fusion protein leads to constitutive activation of down-stream signaling pathways, including Ras, Jak/Stat and PI-3 kinase. In rare cases the BCR/ABL fusion gene is located on chromosomal sites other than the Ph chromosome.
Fluorescence in situ Hybridization (FISH) allows for the identification of rearrangements that could otherwise not be detected by conventional karyotyping.
探针描述
The SPEC BCR/ABL Dual Color Dual Fusion Probe is a mixture of a green fluorochrome direct labeled BCR probe spanning the minor and major breakpoint cluster of the BCR gene and an orange fluorochrome direct labeled ABL probe spanning the breakpoint region of the ABL gene.
结果
In a normal interphase nucleus, two orange and two green signals are expected. A reciprocal translocation involving two breakpoints splits the two signals and generates a fusion signal on each of the chromosomes involved. The chromosomal regions which are not translocated are indicated by the single orange respectively green signal.
采购指南：
联系电话：010-64484022、64484033；400-699-7909
Z-2111-200 ZytoLight SPEC BCR/ABL Dual Color Dual Fusion Probe / 20 (200 μl)
相关产品
Z-2028-20 ZytoLight FISH-Tissue Implementation Kit 20
Incl. Heat Pretreatment Solution Citric, 500 ml; Pepsin Solution, 4 ml; Wash Buffer SSC, 500 ml; 25x Wash Buffer A, 100 ml; DAPI Antifade-Solution, 0.8 ml
