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北京阅微基因技术有限公司
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成立日期:2007
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产品名称:文库定量试剂盒/KAPA Library Quantification Kit

所属分类:其它

浏览:6510

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KAPA Library Quantification Kit  

 

 

 

   现在普通的进行DNA文库定量的方法都比较复杂、昂贵并且不可靠。无论是使用emPCR或bPCR,对模板的精确定量都是至关重要的。新一代测序(NGS)技术中使用的文库定量方法也有许多缺点,在反应前要用电泳或分光光度计法检测总DNA的浓度,依据适当的PCR扩增中DNA的浓度来优化基因簇密度或微珠富集率。这种方法灵敏度低,样品要精确到毫微克,所以不适合高吞吐量的定量PCR。
 
 
   KAPA Library Quantification Kits 包括IIIumina Genome Analyzer , Roche 454 Titanium series 和Roche 454 FLX series,有准确、可靠的DNA 浓度标准和定量PCR 试剂,包含一种新的DNA 聚合酶,该酶是用分子进化方法制造的适用于使用SYBR®Green I 染料的定量PCR。试剂盒中都包含5mL SYBR® FAST qPCR Master Mix(2×), 1mL Primer Premix, 6×80μL DNA Quantification Standards。试剂盒中含有引物和DNA浓度标样,包含可以用于不同DNA测序平台和定量PCR仪器的定量PCR试剂。Primer Premix和DNA Quantification Standards可以单独购买。
 
KAPALibrary Quantification Kits的应用:
  所有libraries 的定量
  不同浓度libraries 片段的定量
  GC 含量变化范围较大的libraries 片段的定量
  片段大小在50-1000bpLibraries 的定量
  可以很好的用于第二代序列定量分析中的PCR-competent DNA 的定量、低浓度libraries的定量,并可以使用自动移液处理。
 
KAPALibrary Quantification Kits的特点:
l.KAPA Library Quantification Kits 不需要昂贵且耗时的滴定,并且形成了一种高效的流线型的操作流程。(下图)

 
 
 
 
 
 
2.精确的定量使得基因簇密度均一
下图:使用KAPA LibraryQuantification Kits 前(左)和使用KAPA LibraryQuantification Kits 之后(右)的基因簇密度

 
 
 
 
3.KAPA Library Quantification Kits 扩增效率高
下图: 使用KAPALibrary Quantification Kits 之前的片段大小分布(灰色)和使用KAPA LibraryQuantification Kits(蓝色)、competitor S ( 红色)competitor F(橙色)之后的片段大小分布

 
下图,对 GC 含量变化范围大的扩增子能精确定量

 
 
 
4.KAPA Library Quantification Kits 能够准确定量并且重复性好(下图)

订购信息:
Code
Description
Kit Contents
KK4800
DNA Quantification Standards and Primer Premix Kit/Roche 454 FLX
Kits contain 1 mL Primer Premix and 6 x 80 µL DNA Quantification Standards optimized for the Roche 454 FLX.
KK4801
Primer Premix/ Roche 454 FLX
1 mL Primer Premix optimized for the Roche 454 FLX.
KK4802
DNA Quantification Standards and Primer Premix Kit/ Roche 454 Titanium
Kits contain 1 mL Primer Premix and 6 x 80 µL DNA Quantification Standards optimized for the Roche 454 Titanium.
KK4803
Primer Premix/ Roche 454 Titanium
1 ml Primer Premix optimized for the Roche 454 Titanium.
KK4806
DNA Quantification Standards and Primer Premix Kit/ABI SOLiD
Kits contain 1 mL Primer Premix and 6 x 80 µL DNA Quantification Standards optimized for the ABI SOLiD.
KK4807
Primer Premix/ABI SOLiD
1 mL Primer Premix optimized for the ABI SOLiD.
KK4808
DNA Quantification Standards and Primer Premix Kit/ Illumina GA
Kits contain 1 mL Primer Premix and 6 x 80 µL DNA Quantification Standards optimized for the Illumina Genome Analyzer.
KK4809
Primer Premix/ Illumina GA
1 ml Primer Premix optimized for the Illumina Genome Analyzer.
KK4820
Library Quantification Kit/ Roche 454 FLX/ Universal
Kits contain 5 mL KAPA SYBR® FAST Universal qPCR Master Mix (2X), 200 µL ROX High (50X), 200 µL ROX Low (50X), 1 mL Primer Premix, and 6 x 80 µL DNA Quantification Standards. Primer Premix and DNA Standards are optimized for the Roche 454 FLX.
KK4821
Library Quantification Kit/ Roche 454 Titanium/ Universal
Kits contain 5 mL KAPA SYBR® FAST Universal qPCR Master Mix (2X), 200 µL ROX High (50X), 200 µL ROX Low (50X), 1 mL Primer Premix, and 6 x 80 µL DNA Quantification Standards. Primer Premix and DNA Standards are optimized for the Roche 454 Titanium.
KK4823
Library Quantification Kit/ABI SOLiD/Universal
Kits contain 5 mL KAPA SYBR® FAST Universal qPCR Master Mix (2X), 200 µL ROX High (50X), 200 µL ROX Low (50X), 1 mL Primer Premix, and 6 x 80 µL DNA Quantification Standards. Primer Premix and DNA Standards are optimized for the ABI SOLiD.
KK4824
Library Quantification Kit/ Illumina GA/ Universal
Kits contain 5 mL KAPA SYBR® FAST Universal qPCR Master Mix (2X), 200 µL ROX High (50X), 200 µL ROX Low (50X), 1 mL Primer Premix, and 6 x 80 µL DNA Quantification Standards. Primer Premix and DNA Standards are optimized for the Illumina Genome Analyzer.
KK4830
Library Quantification Kit/ Roche 454 FLX/ ABI Prism
Kits contain 5 mL KAPA SYBR® FAST ABI Prism® qPCR Master Mix (2X), 1 mL Primer Premix, and 6 x 80 µL DNA Quantification Standards. Primer Premix and DNA Quantification Standards are optimized for the Roche 454 FLX.
KK4831
Library Quantification Kit/ Roche 454 Titanium/ ABI Prism
Kits contain 5 mL KAPA SYBR® FAST ABI Prism® qPCR Master Mix (2X), 1 mL Primer Premix, and 6 x 80 µL DNA Quantification Standards. Primer Premix and DNA Quantification Standards are optimized for the Roche 454 Titanium.
KK4833
Library Quantification Kit/ABI SOLiD/ABI Prism
Kits contain 5 mL KAPA SYBR® FAST ABI Prism® qPCR Master Mix (2X), 1 mL Primer Premix, and 6 x 80 µL DNA Quantification Standards. Primer Premix and DNA Quantification Standards are optimized for the ABI SOLiD.
KK4835
Library Quantification Kit/ Illumina GA/ ABI Prism
Kits contain 5 mL KAPA SYBR® FAST ABI Prism® qPCR Master Mix (2X), 1 mL Primer Premix, and 6 x 80 µL DNA Quantification Standards. Primer Premix and DNA Quantification Standards are optimized for the Illumina Genome Analyzer.
KK4840
Library Quantification Kit/ Roche 454 FLX/ Bio-Rad iCycler
Kits contain 5 mL KAPA SYBR® FAST Bio-Rad iCycler™ qPCR Master Mix (2X), 1 mL Primer Premix, and 6 x 80 µL DNA Quantification Standards. Primer Premix and DNA Quantification Standards are optimized for the Roche 454 FLX.
KK4841
Library Quantification Kit/ Roche 454 Titanium/ Bio-Rad iCycler
Kits contain 5 mL KAPA SYBR® FAST Bio-Rad iCycler™ qPCR Master Mix (2X), 1 mL Primer Premix, and 6 x 80 µL DNA Quantification Standards. Primer Premix and DNA Quantification Standards are optimized for the Roche 454 Titanium.
KK4843
Library Quantification Kit/ABI SOLiD/Bio-Rad iCycler
Kits contain 5 mL KAPA SYBR® FAST Bio-Rad iCycler™ qPCR Master Mix (2X), 1 mL Primer Premix, and 6 x 80 µL DNA Quantification Standards. Primer Premix and DNA Quantification Standards are optimized for the ABI SOLiD.
KK4844
Library Quantification Kit/ Illumina GA/ Bio-Rad iCycler
Kits contain 5 mL KAPA SYBR® FAST Bio-Rad iCycler™ qPCR Master Mix (2X), 1 mL Primer Premix, and 6 x 80 µL DNA Quantification Standards. Primer Premix and DNA Quantification Standards are optimized for the Illumina Genome Analyzer.
KK4850
Library Quantification Kit/ Roche 454 FLX/ Roche LC 480
Kits contain 5 mL KAPA SYBR® FAST qPCR Master Mix (2X) optimized for Roche LightCycler® 480, 1 mL Primer Premix, and 6 x 80 µL DNA Quantification Standards. Primer Premix and DNA Quantification Standards are optimized for the Roche 454 FLX.
KK4851
Library Quantification Kit/ Roche 454 Titanium/ Roche LC 480
Kits contain 5 mL KAPA SYBR® FAST qPCR Master Mix (2X) optimized for Roche LightCycler® 480, 1 mL Primer Premix, and 6 x 80 µL DNA Quantification Standards. Primer Premix and DNA Quantification Standards are optimized for the Roche 454 Titanium.
KK4853
Library Quantification Kit/ABI SOLiD/Roche LC 480
Kits contain 5 mL KAPA SYBR® FAST qPCR Master Mix (2X) optimized for Roche LightCycler® 480, 1 mL Primer Premix, and 6 x 80 µL DNA Quantification Standards. Primer Premix and DNA Quantification Standards are optimized for the ABI SOLiD.
KK4854
Library Quantification Kit/ Illumina GA/ Roche LC 480
Kits contain 5 mL KAPA SYBR® FAST qPCR Master Mix (2X) optimized for Roche LightCycler® 480, 1 mL Primer Premix, and 6 x 80 µL DNA Quantification Standards. Primer Premix and DNA Quantification Standards are optimized for the Illumina Genome Analyzer.
 
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